The blood cells and haemopoiesis of Diplodus sargus L: Haematological values, cytochemistry and leukocytes' response to vaccine stimulation

The treatment with antipiogenic and trivalent antiviral vaccines evokes in sargus' blood an increase of the neutrophilic granulocytes and an activation of the lymphocytes. By using cytochemical reactions for acid phosphatase and aliesterase, a population of lymphocytes, amounting to about 25%, which displays positive reactions has been identified. The immunocytochemical reactions used to identify the mammalian T and B-lymphocytes, such as resetting test, immune localization of CD (Cluster of differentiation) and of sig, were performed with the aim of better classifying the lymphocytes of sargus, even though the use of mammalian monoclonal CD antibodies cannot be considered a certain proof of such a presence. Moreover, cytochemical reactions have revealed the presence of lysosomal enzymes in neutrophils and the presence of peroxidase which characterize the eosinophils. Hemopoiesis takes place in the spleen and in the lymphomieloid tissue of the kidney.