Recently, microRNAs (miRNAs) were identified able to inhibit the expression of the Cystic Fibrosis Transmembrane Regulator (CFTR) disease-gene of Cystic Fibrosis (CF) and CFTR-Related Disorders (CFTR-RD). This study shows the use of peptide nucleic acids (PNAs) as inhibitors of miR-509-3p, one of the CFTR regulating miRNAs, by reverting the expression of luciferase gene containing the 3 'UTR of CFTR gene.
Design, synthesis and biochemical investigation, by in vitro luciferase reporter system, of peptide nucleic acids as new inhibitors of miR-509-3p involved in the regulation of cystic fibrosis disease-gene expression / Amato, Felice; Tomaiuolo, Rossella; Borbone, Nicola; Ausilia, Elce; Amato, Jussara; D'Errico, Stefano; DE ROSA, Giuseppe; Mayol, Laura; Piccialli, Gennaro; Oliviero, Giorgia; Castaldo, Giuseppe. - In: MEDCHEMCOMM. - ISSN 2040-2503. - 5:1(2014), pp. 68-71. [10.1039/C3MD00257H]
Design, synthesis and biochemical investigation, by in vitro luciferase reporter system, of peptide nucleic acids as new inhibitors of miR-509-3p involved in the regulation of cystic fibrosis disease-gene expression
AMATO, FELICE;TOMAIUOLO, ROSSELLA;BORBONE, NICOLA;AMATO, JUSSARA;D'ERRICO, STEFANO;DE ROSA, GIUSEPPE;MAYOL, LAURA;PICCIALLI, GENNARO;OLIVIERO, GIORGIA;CASTALDO, GIUSEPPE
2014
Abstract
Recently, microRNAs (miRNAs) were identified able to inhibit the expression of the Cystic Fibrosis Transmembrane Regulator (CFTR) disease-gene of Cystic Fibrosis (CF) and CFTR-Related Disorders (CFTR-RD). This study shows the use of peptide nucleic acids (PNAs) as inhibitors of miR-509-3p, one of the CFTR regulating miRNAs, by reverting the expression of luciferase gene containing the 3 'UTR of CFTR gene.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
