We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein.
AN INTEGRATED STRUCTURAL AND COMPUTATIONAL STUDY ON THE THERMOSTABILITY OF TWO THIOREDOXIN MUTANTS FROM ALICYCLOBACILLUS ACIDOCALDARIUS / Bartolucci, Simonetta; Galdiero, Stefania; R., Improta; V. MENCHISE C., Pedone; E., Pedone; M., Saviano. - In: JOURNAL OF BACTERIOLOGY. - ISSN 0021-9193. - STAMPA. - 185:14(2003), pp. 4285-4289. [10.1128/JB.185.14.4285–4289.2003]
AN INTEGRATED STRUCTURAL AND COMPUTATIONAL STUDY ON THE THERMOSTABILITY OF TWO THIOREDOXIN MUTANTS FROM ALICYCLOBACILLUS ACIDOCALDARIUS
BARTOLUCCI, SIMONETTA;GALDIERO, STEFANIA;
2003
Abstract
We report a crystallographic and computational analysis of two mutant forms of the Alicyclobacillus acidocaldarius thioredoxin (BacTrx) done in order to evaluate the contribution of two specific amino acids to the thermostability of BacTrx. Our results suggest that the thermostability of BacTrx may be modulated by mutations affecting the overall electrostatic energy of the protein.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
