The aimof this studywas to assess the phytotoxic potential of Cleome arabica L, as well as to isolate the main bioactive compounds. Phytotoxicity was evaluated on germination and seedling growth of Lactuca sativa, Raphanus sativus, Peganum harmala and Silybum marianum, through testing aqueous and organic extracts of different C. arabica organs (roots, shoots, siliquae and seeds). Results showed that siliquae methanol extract caused the greatest negative effect on lettuce germination and growth. For the bioactive subfractions (petroleum ether, ethyl acetate andmethanol–water), the ethyl acetate induced highly significant reduction, showing 100% inhibition of lettuce growth at 6 g/L. The bioactive ethyl acetate subfraction was chromatographed and subjected to NMR techniques. Based on bio-guided chromatographic fractionation, five bioactive allelochemical compounds were isolated from ethyl acetate extract of siliquae of C. arabica. The most inhibitory compound on lettuce seedling growth was elucidated as 11-α-acetylbrachy-carpone-22(23)-ene.
Phytotoxic activity of Cleome arabica L. and its principal discovered active compounds / Ladhari, A.; Omezzine, F.; DELLA GRECA, Marina; Zarrelli, Armando; Zuppolini, Simona; Haouala, R.. - In: SOUTH AFRICAN JOURNAL OF BOTANY. - ISSN 0254-6299. - 88:-(2013), pp. 341-351. [10.1016/j.sajb.2013.08.016]
Phytotoxic activity of Cleome arabica L. and its principal discovered active compounds
DELLA GRECA, MARINA;ZARRELLI, ARMANDO;ZUPPOLINI, SIMONA;
2013
Abstract
The aimof this studywas to assess the phytotoxic potential of Cleome arabica L, as well as to isolate the main bioactive compounds. Phytotoxicity was evaluated on germination and seedling growth of Lactuca sativa, Raphanus sativus, Peganum harmala and Silybum marianum, through testing aqueous and organic extracts of different C. arabica organs (roots, shoots, siliquae and seeds). Results showed that siliquae methanol extract caused the greatest negative effect on lettuce germination and growth. For the bioactive subfractions (petroleum ether, ethyl acetate andmethanol–water), the ethyl acetate induced highly significant reduction, showing 100% inhibition of lettuce growth at 6 g/L. The bioactive ethyl acetate subfraction was chromatographed and subjected to NMR techniques. Based on bio-guided chromatographic fractionation, five bioactive allelochemical compounds were isolated from ethyl acetate extract of siliquae of C. arabica. The most inhibitory compound on lettuce seedling growth was elucidated as 11-α-acetylbrachy-carpone-22(23)-ene.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
