Aflatoxins are natural contaminants frequently found both in food and feed. Many of them exert immunomodulatory properties in mammals; therefore, the aim of the current study was to investigate immune-effects of AFB1, AFB2, AFM1 and AFM2, alone and differently combined, in J774A.1 murine macrophages. MTT assay showed that AFB1, alone and combined with AFB2, possess antiproliferative activity only at the highest concentration; such effect was not shown by their hydroxylated metabolites. AFM1 and AFM2, respectively. However, the immunotoxic effects of the aflatoxins evaluated in the current study may be due to the inhibition of production of active oxygen metabolites such as NO. Cytofluorimetric assay in macrophages exposed to aflatoxins (10-100 mu M) revealed that their cytoxicity is not related to apoptotic pathways. Nevertheless, a significant increase of the 5 phase cell population accompanied by a decrease in G0/G1 phase cell population was observed after AFB1 treatment.
Modulation of macrophage activity by aflatoxins B1 and B2 and their metabolites aflatoxins M1 and M2 / Bianco, G.; Russo, R.; Marzocco, S.; Velotto, Salvatore; Autore, G.; Severino, Lorella. - In: TOXICON. - ISSN 0041-0101. - 59:6(2012), pp. 644-650.
Modulation of macrophage activity by aflatoxins B1 and B2 and their metabolites aflatoxins M1 and M2
VELOTTO, SALVATORE;SEVERINO, LORELLA
2012
Abstract
Aflatoxins are natural contaminants frequently found both in food and feed. Many of them exert immunomodulatory properties in mammals; therefore, the aim of the current study was to investigate immune-effects of AFB1, AFB2, AFM1 and AFM2, alone and differently combined, in J774A.1 murine macrophages. MTT assay showed that AFB1, alone and combined with AFB2, possess antiproliferative activity only at the highest concentration; such effect was not shown by their hydroxylated metabolites. AFM1 and AFM2, respectively. However, the immunotoxic effects of the aflatoxins evaluated in the current study may be due to the inhibition of production of active oxygen metabolites such as NO. Cytofluorimetric assay in macrophages exposed to aflatoxins (10-100 mu M) revealed that their cytoxicity is not related to apoptotic pathways. Nevertheless, a significant increase of the 5 phase cell population accompanied by a decrease in G0/G1 phase cell population was observed after AFB1 treatment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
