In this work the thermophilic trehalose/maltose-binding protein from Thermococcus litoralis is presented as a probe for the design of a high stable fluorescence biosensor for glucose. In particular, we show the possibility of modulating the protein specificity by changing temperature. In addition to glucose sensing, we also report on the possibility of utilizing odorant-binding proteins as a probe for the development of optical sensors for analytes of environmental interests.
New trends in bio/nanotechnology: stable proteins as advanced molecular tools for health and environment / M., Staiano; M., Baldassarre; M., Esposito; Apicella, Elisa; R., Vitale; V., Aurilia; S., D'Auria. - In: ENVIRONMENTAL TECHNOLOGY. - ISSN 1479-487X. - 31:8-9(2010), pp. 935-942. [10.1080/09593331003639575]
New trends in bio/nanotechnology: stable proteins as advanced molecular tools for health and environment
APICELLA, ELISA;
2010
Abstract
In this work the thermophilic trehalose/maltose-binding protein from Thermococcus litoralis is presented as a probe for the design of a high stable fluorescence biosensor for glucose. In particular, we show the possibility of modulating the protein specificity by changing temperature. In addition to glucose sensing, we also report on the possibility of utilizing odorant-binding proteins as a probe for the development of optical sensors for analytes of environmental interests.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
