The delivery of peptide nucleic acids (PNAs) to cells is a very challenging task. We report here that a liposomal formulation composed of egg PC/cholesterol/DSPE-PEG2000 can be loaded, according to different encapsulation techniques, with PNA or fluorescent PNA oligomers. PNA loaded liposomes efficiently and quickly promote the uptake of a PNA targeting the microRNA miR-210 in human erythroleukemic K562 cells. By using this innovative delivery system for PNA, down-regulation of miR-210 is achieved at a low PNA concentration.
Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery / Avitabile, Concetta; Accardo, Antonella; Ringhieri, Paola; Morelli, Giancarlo; Saviano, Michele; Montagner, Giulia; Fabbri, Enrica; Gallerani, Eleonora; Gambari, Roberto; Romanelli, Alessandra. - In: BIOCONJUGATE CHEMISTRY. - ISSN 1043-1802. - 26:8(2015), pp. 1533-41-1541. [10.1021/acs.bioconjchem.5b00156]
Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery
AVITABILE, CONCETTA;ACCARDO, ANTONELLA;RINGHIERI, PAOLA;MORELLI, GIANCARLO;SAVIANO, MICHELE;ROMANELLI, ALESSANDRA
2015
Abstract
The delivery of peptide nucleic acids (PNAs) to cells is a very challenging task. We report here that a liposomal formulation composed of egg PC/cholesterol/DSPE-PEG2000 can be loaded, according to different encapsulation techniques, with PNA or fluorescent PNA oligomers. PNA loaded liposomes efficiently and quickly promote the uptake of a PNA targeting the microRNA miR-210 in human erythroleukemic K562 cells. By using this innovative delivery system for PNA, down-regulation of miR-210 is achieved at a low PNA concentration.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
