Adipose tissue-derived mesenchymal stem cells (Ad-MSC) and platelet derivatives have been used alone or in combination to achieve regeneration of injured tissues. We have tested the effect of platelet-rich plasma (PRP) on Ad-MSC and adipocyte function. PRP increased Ad-MSC viability, proliferation rate and G1-S cell cycle progression, by at least 7-, 2-, and 2.2-fold, respectively, and reduced caspase 3 cleavage. Higher PRP concentrations or PRPs derived from individuals with higher platelet counts were more effective in increasing Ad-MSC growth. PRP also accelerated cell migration by at least 1.5-fold. However, PRP did not significantly affect mature adipocyte viability, differentiation and expression levels of PPAR-γ and AP-2 mRNAs, while it increased leptin production by 3.5-fold. Interestingly, PRP treatment of mature adipocytes also enhanced the release of Interleukin (IL)-6, IL-8, IL-10, Interferon-γ, and Vascular Endothelial Growth Factor. Thus, data are consistent with a stimulatory effect of platelet derivatives on Ad-MSC growth and motility. Moreover, PRP did not reduce mature adipocyte survival and increased the release of pro-angiogenic factors, which may facilitate tissue regeneration processes.

Platelet - Rich Plasma Increases Growth and Motility of Adipose Tissue - Derived Mesenchymal Stem Cells and Controls Adipocyte Secretory Function / D'Esposito, V; Passaretti, F; Perruolo, G; Ambrosio, Mr; Valentino, R; Oriente, F; Raciti, Ga; Nigro, C; Miele, C; Sammartino, G; Beguinot, F; Formisano, P. - In: JOURNAL OF CELLULAR BIOCHEMISTRY. - ISSN 0730-2312. - 116:10(2015), pp. 2408-2418. [10.1002/jcb.25235]

Platelet - Rich Plasma Increases Growth and Motility of Adipose Tissue - Derived Mesenchymal Stem Cells and Controls Adipocyte Secretory Function

D'Esposito V;Perruolo G;RACITI GA;Sammartino G;Beguinot F;Formisano P
2015

Abstract

Adipose tissue-derived mesenchymal stem cells (Ad-MSC) and platelet derivatives have been used alone or in combination to achieve regeneration of injured tissues. We have tested the effect of platelet-rich plasma (PRP) on Ad-MSC and adipocyte function. PRP increased Ad-MSC viability, proliferation rate and G1-S cell cycle progression, by at least 7-, 2-, and 2.2-fold, respectively, and reduced caspase 3 cleavage. Higher PRP concentrations or PRPs derived from individuals with higher platelet counts were more effective in increasing Ad-MSC growth. PRP also accelerated cell migration by at least 1.5-fold. However, PRP did not significantly affect mature adipocyte viability, differentiation and expression levels of PPAR-γ and AP-2 mRNAs, while it increased leptin production by 3.5-fold. Interestingly, PRP treatment of mature adipocytes also enhanced the release of Interleukin (IL)-6, IL-8, IL-10, Interferon-γ, and Vascular Endothelial Growth Factor. Thus, data are consistent with a stimulatory effect of platelet derivatives on Ad-MSC growth and motility. Moreover, PRP did not reduce mature adipocyte survival and increased the release of pro-angiogenic factors, which may facilitate tissue regeneration processes.
2015
Platelet - Rich Plasma Increases Growth and Motility of Adipose Tissue - Derived Mesenchymal Stem Cells and Controls Adipocyte Secretory Function / D'Esposito, V; Passaretti, F; Perruolo, G; Ambrosio, Mr; Valentino, R; Oriente, F; Raciti, Ga; Nigro, C; Miele, C; Sammartino, G; Beguinot, F; Formisano, P. - In: JOURNAL OF CELLULAR BIOCHEMISTRY. - ISSN 0730-2312. - 116:10(2015), pp. 2408-2418. [10.1002/jcb.25235]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/635458
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