Liposomal formulations composed of phospholipids with different unsaturation degrees, head groupsand at different cholesterol content have been tested for the encapsulation of Peptide Nucleic Acid (PNA)oligomers. The best loading capability (177 g, ER% = 87.2) was obtained for pure liposomes of phos-phatidylglycerol (DOPG) with negatively charged head group. The insertion of a 10–20% of cholesterol inDOPG based liposomes provides a slight decrease (∼160 g) of the PNA loading. On the other hand, thecholesterol addition (20–30%) slows down the PNA’s release (∼27%) in fetal bovine serum from the lipo-somal formulation. Based on the encapsulation and the release properties, PEGylated DOPG liposomeswith a percentage of cholesterol of 10–20% are the optimal formulation for the loading of PNA-a210.
The influence of liposomal formulation on the incorporation and retention of PNA oligomers / Ringhieri, Paola; Avitabile, Concetta; Saviano, Michele; Morelli, Giancarlo; Romanelli, Alessandra; Accardo, Antonella. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 0927-7765. - 145:(2016), pp. 462-469. [10.1016/j.colsurfb.2016.05.034]
The influence of liposomal formulation on the incorporation and retention of PNA oligomers
RINGHIERI, PAOLA;AVITABILE, CONCETTA;SAVIANO, MICHELE;MORELLI, GIANCARLO;ROMANELLI, ALESSANDRA;ACCARDO, ANTONELLA
2016
Abstract
Liposomal formulations composed of phospholipids with different unsaturation degrees, head groupsand at different cholesterol content have been tested for the encapsulation of Peptide Nucleic Acid (PNA)oligomers. The best loading capability (177 g, ER% = 87.2) was obtained for pure liposomes of phos-phatidylglycerol (DOPG) with negatively charged head group. The insertion of a 10–20% of cholesterol inDOPG based liposomes provides a slight decrease (∼160 g) of the PNA loading. On the other hand, thecholesterol addition (20–30%) slows down the PNA’s release (∼27%) in fetal bovine serum from the lipo-somal formulation. Based on the encapsulation and the release properties, PEGylated DOPG liposomeswith a percentage of cholesterol of 10–20% are the optimal formulation for the loading of PNA-a210.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
