Many efforts are being made in the design and engineering of metalloenzymes with catalytic properties fulfilling the needs of practical applications. Progress in this field has recently been accelerated by advances in computational, molecular and structural biology. This review article focuses on the recent examples of oxygen-activating metalloenzymes, developed through the strategies of de novo design, miniaturization processes and protein redesign. Considerable progress in these diverse design approaches has produced many metal-containing biocatalysts able to adopt the functions of native enzymes or even novel functions beyond those found in Nature.
Design and engineering of artificial oxygen-activating metalloenzymes / Nastri, Flavia; Chino, Marco; Maglio, Ornella; Bhagi Damodaran, Ambika; Lu, Yi; Lombardi, Angelina. - In: CHEMICAL SOCIETY REVIEWS. - ISSN 0306-0012. - 45:18(2016), pp. 5020-5054. [10.1039/c5cs00923e]
Design and engineering of artificial oxygen-activating metalloenzymes
NASTRI, FLAVIA;CHINO, MARCO;MAGLIO, ORNELLA;LOMBARDI, ANGELINA
2016
Abstract
Many efforts are being made in the design and engineering of metalloenzymes with catalytic properties fulfilling the needs of practical applications. Progress in this field has recently been accelerated by advances in computational, molecular and structural biology. This review article focuses on the recent examples of oxygen-activating metalloenzymes, developed through the strategies of de novo design, miniaturization processes and protein redesign. Considerable progress in these diverse design approaches has produced many metal-containing biocatalysts able to adopt the functions of native enzymes or even novel functions beyond those found in Nature.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
