The second-generation Pt anticancer agent carboplatin (CBDCA) was encapsulated within the apo horse spleen ferritin (AFt) nanocage, and the X-ray structure of the drug-loaded protein was refined at 1.49 Å resolution. Two Pt binding sites, different from the one observed in the cisplatin-encapsulated AFt, were identified in Ft subunits by inspection of anomalous electron density maps at two wavelengths and difference Fourier electron density maps, which provide the necessary sensitivity to discriminate between Pt from CBDCA and Cd ions that are present in the crystallization conditions. Pt centers coordinate to the NE2 atom of His49 and to the NE2 atom of His132, both on the inner surface of the Ft nanocage.
X-ray Structure of the Carboplatin-Loaded Apo-Ferritin Nanocage / Pontillo, Nicola; Ferraro, Giarita; Helliwell, John R; Amoresano, Angela; Merlino, Antonello. - In: ACS MEDICINAL CHEMISTRY LETTERS. - ISSN 1948-5875. - 8:4(2017), pp. 433-437-437. [10.1021/acsmedchemlett.7b00025]
X-ray Structure of the Carboplatin-Loaded Apo-Ferritin Nanocage
FERRARO, GIARITA;AMORESANO, ANGELA;MERLINO, ANTONELLO
2017
Abstract
The second-generation Pt anticancer agent carboplatin (CBDCA) was encapsulated within the apo horse spleen ferritin (AFt) nanocage, and the X-ray structure of the drug-loaded protein was refined at 1.49 Å resolution. Two Pt binding sites, different from the one observed in the cisplatin-encapsulated AFt, were identified in Ft subunits by inspection of anomalous electron density maps at two wavelengths and difference Fourier electron density maps, which provide the necessary sensitivity to discriminate between Pt from CBDCA and Cd ions that are present in the crystallization conditions. Pt centers coordinate to the NE2 atom of His49 and to the NE2 atom of His132, both on the inner surface of the Ft nanocage.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
