A differential sister chromatid exchange (SCE)/cell response was observed between phytohemagglutinin (PHA) and pokeweed (PKW) stimulated blood lymphocytes of goat (Capra hircus L) exposed to final concentrations of 0.1, 0.25, 0.5, 1, 2.5 and 5 pg/mL of BUdR. At 0.1 !g/mL of BUdR, the two mitogens gave very similar SCE/cell responses: the SCE mean values were 3.17 ! 1.93 for PHA and 3.28 ! 1.76 for PKW, and the frequency distributions fit very well the Poisson probability function with both mitogens. For 0.25 vg/mL and increasing BUdR concentrations, SCE/cell rates for pokeweed mitogen were significantly higher than those of PHA. At 5 4g/mL of BUdR, the SCE/cell response was 8.68 ! 3.24 for PKW and 6.96 ! 3.45 for PHA, and the difference was statistically significant (P = 0.0001); for both mitogens the SCE/cell frequency distributions fit the Poisson probability function only by adopting a Poisson ’mixture’ model, which takes into account the presence of two different subpopulations of cells.
Differential sister chromatid exchanges response in phytohemagglutinin and pokeweed stimulated lymphocytes of goat (Capra hircus L.) / Crasto, Antonio; Scarfi I. Burguete, Mr. - In: GENETICS SELECTION EVOLUTION. - ISSN 0999-193X. - 29:(1997), pp. 185-192.
Differential sister chromatid exchanges response in phytohemagglutinin and pokeweed stimulated lymphocytes of goat (Capra hircus L.)
CRASTO, ANTONIO;
1997
Abstract
A differential sister chromatid exchange (SCE)/cell response was observed between phytohemagglutinin (PHA) and pokeweed (PKW) stimulated blood lymphocytes of goat (Capra hircus L) exposed to final concentrations of 0.1, 0.25, 0.5, 1, 2.5 and 5 pg/mL of BUdR. At 0.1 !g/mL of BUdR, the two mitogens gave very similar SCE/cell responses: the SCE mean values were 3.17 ! 1.93 for PHA and 3.28 ! 1.76 for PKW, and the frequency distributions fit very well the Poisson probability function with both mitogens. For 0.25 vg/mL and increasing BUdR concentrations, SCE/cell rates for pokeweed mitogen were significantly higher than those of PHA. At 5 4g/mL of BUdR, the SCE/cell response was 8.68 ! 3.24 for PKW and 6.96 ! 3.45 for PHA, and the difference was statistically significant (P = 0.0001); for both mitogens the SCE/cell frequency distributions fit the Poisson probability function only by adopting a Poisson ’mixture’ model, which takes into account the presence of two different subpopulations of cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
