Obtaining DNA nanostructures with potential applications in drug discovery, diagnostics, and electronics in a simple and affordable way represents one of the hottest topics in nanotechnological and medical sciences. Herein, we report a novel strategy to obtain structurally homogeneous DNA G-wire nanostructures of known length, starting from the short unmodified G-rich oligonucleotide d(5′-CGGT-3′–3′-GGC-5′) (1) incorporating a 3’–3′ inversion of polarity site. The reported approach allowed us to obtain long G-wire assemblies through 5′–5′ π–π stacking interactions in between the tetramolecular G-quadruplex building blocks that form when 1 is annealed in the presence of potassium ions. Our results expand the repertoire of synthetic methodologies to obtain new tailored DNA G-wire nanostructures.
Self-Assembly of G-Rich Oligonucleotides Incorporating a 3′-3′ Inversion of Polarity Site: A New Route Towards G-Wire DNA Nanostructures / Oliviero, Giorgia; D'Errico, Stefano; Pinto, Brunella; Nici, Fabrizia; Dardano, Principia; Rea, Ilaria; DE STEFANO, Luca; Mayol, Luciano; Piccialli, Gennaro; Borbone, Nicola. - In: CHEMISTRYOPEN. - ISSN 2191-1363. - 6:4(2017), pp. 599-605. [10.1002/open.201700024]
Self-Assembly of G-Rich Oligonucleotides Incorporating a 3′-3′ Inversion of Polarity Site: A New Route Towards G-Wire DNA Nanostructures
OLIVIERO, GIORGIA;D'ERRICO, STEFANO;PINTO, BRUNELLA;NICI, FABRIZIA;DARDANO, PRINCIPIA;REA, ILARIA;DE STEFANO, Luca;MAYOL, LUCIANO;PICCIALLI, GENNARO;BORBONE, NICOLA
2017
Abstract
Obtaining DNA nanostructures with potential applications in drug discovery, diagnostics, and electronics in a simple and affordable way represents one of the hottest topics in nanotechnological and medical sciences. Herein, we report a novel strategy to obtain structurally homogeneous DNA G-wire nanostructures of known length, starting from the short unmodified G-rich oligonucleotide d(5′-CGGT-3′–3′-GGC-5′) (1) incorporating a 3’–3′ inversion of polarity site. The reported approach allowed us to obtain long G-wire assemblies through 5′–5′ π–π stacking interactions in between the tetramolecular G-quadruplex building blocks that form when 1 is annealed in the presence of potassium ions. Our results expand the repertoire of synthetic methodologies to obtain new tailored DNA G-wire nanostructures.File | Dimensione | Formato | |
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