The aim of this work is to identify the cisplatin binding sites on human H-chain ferritin. High-resolution X-ray crystallography reveals that cisplatin binds four distinct protein sites, that is, the side chains of His136 and Lys68, the side chain of His105, the side chain of Cys90 and the side chain of Cys102. These Pt binding sites are compared with those observed for the adduct that cisplatin forms upon encapsulation within horse spleen L-chain ferritin (87% identity with human L-chain ferritin).
Cisplatin Binding Sites in Human H-Chain Ferritin / Ferraro, Giarita; Ciambellotti, Silvia; Messori, Luigi; Merlino, Antonello. - In: INORGANIC CHEMISTRY. - ISSN 0020-1669. - 56:15(2017), pp. 9064-9070. [10.1021/acs.inorgchem.7b01072]
Cisplatin Binding Sites in Human H-Chain Ferritin
FERRARO, GIARITA;MERLINO, ANTONELLO
2017
Abstract
The aim of this work is to identify the cisplatin binding sites on human H-chain ferritin. High-resolution X-ray crystallography reveals that cisplatin binds four distinct protein sites, that is, the side chains of His136 and Lys68, the side chain of His105, the side chain of Cys90 and the side chain of Cys102. These Pt binding sites are compared with those observed for the adduct that cisplatin forms upon encapsulation within horse spleen L-chain ferritin (87% identity with human L-chain ferritin).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
