The lncRNA HOTAIR was regulated by polyacrylic acid resins in human gingival fibroblasts

Although, the studies about the genotoxic and cytotoxic effects of methacrylate resins have increased considerably, the understanding of molecular regulatory basis is still poorly understood. HOTAIR is a non-coding RNA related to several diseases; originally, it was found in colorectal, gastric cancer and hepatoma. Moreover, HOTAIR is one of the most important regulator of the Epithelial-Mesenchymal Transition process. The aim of this paper was to investigate the ability of three commercial acid resins (Unifast, JetKit and Duralay) to control the HOTAIR gene expression. The cytotoxic activity in HGFs, measured by MTT assay, was always conditioned by high concentrations of the resins. On the other hand, the proliferation rate does not change in HGFs treated with Unifast and Duralay, while Jet Kit is a negative regulator of the cell proliferation. The HOTAIR gene expression was regulated in HGFs treated with Unifast and Duralay, while JetKit did not induce change in charge of HOTAIR expression. In this study, the ability of different commercial polyacrylic acid resins to regulate lncHOTAIR gene has been demonstrated for the first time. Moreover, no data have previously been reported about the HOTAIR gene expression in HGFs. Several experimental evidences identify HOTAIR as a crucial regulator of EMT; pathologically, any alteration of EMT process leads to several diseases. Particularly, overgrowth of gingival epithelium is due to the deregulation of EMT specific markers. In conclusion, our results could lead to better understanding the role of HOTAIR in different gingival pathologies related to the EMT deregulation.