DNA G-triplex structures are interesting intermediates in the folding process of G-quadruplexes. That means G-triplexes might represent convenient pharmacological targets for gene expression modulation. However, the literature comes with little knowledge concerning the effects of epigenetic or other chemical modifications on their physical–chemical properties. Herein we explore the biophysical consequences of substituting 5-hydroxymethyl-2′-deoxyuridine to thymidine in a stable G-triplex-forming telomeric sequence by combining circular dichroism, differential scanning calorimetry and gel electrophoresis
G-triplex stability in human telomeric DNA with epigenetic modification/oxidative damage to thymine / Caterino, M.; Virgilio, A.; Esposito, V.; Petraccone, L.; Galeone, A.; Giancola, C.. - In: JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY. - ISSN 1388-6150. - 134:(2018), pp. 1-7. [10.1007/s10973-018-7355-z]
G-triplex stability in human telomeric DNA with epigenetic modification/oxidative damage to thymine
Caterino, M.Co-primo
;Virgilio, A.Co-primo
;Esposito, V.Secondo
;Petraccone, L.;Galeone, A.
Penultimo
;Giancola, C.
Ultimo
2018
Abstract
DNA G-triplex structures are interesting intermediates in the folding process of G-quadruplexes. That means G-triplexes might represent convenient pharmacological targets for gene expression modulation. However, the literature comes with little knowledge concerning the effects of epigenetic or other chemical modifications on their physical–chemical properties. Herein we explore the biophysical consequences of substituting 5-hydroxymethyl-2′-deoxyuridine to thymidine in a stable G-triplex-forming telomeric sequence by combining circular dichroism, differential scanning calorimetry and gel electrophoresisI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
