Molecular testing of cytological lung cancer specimens includes, beyond epidermal growth factor receptor (EGFR), emerging predictive/prognostic genomic biomarkers such as Kirsten rat sarcoma viral oncogene homolog (KRAS), neuroblastoma RAS viral [v-ras] oncogene homolog (NRAS), B-Raf proto-oncogene, serine/threonine kinase (BRAF), and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α (PIK3CA). Next-generation sequencing (NGS) and other multigene mutational assays are suitable for cytological specimens, including smears. However, the current literature reflects single-institution studies rather than multicenter experiences.
Consistency and reproducibility of next-generation sequencing and other multigene mutational assays: A worldwide ring trial study on quantitative cytological molecular reference specimens / Malapelle, U; Mayo-de-Las-Casas, C; Molina-Vila, Ma; Rosell, R; Savic, S; Bihl, M; Bubendorf, L; Salto-Tellez, M; de Biase, D; Tallini, G; Hwang, Dh; Sholl, Lm; Luthra, R; Weynand, B; Vander Borght, S; Missiaglia, E; Bongiovanni, M; Stieber, D; Vielh, P; Schmitt, F; Rappa, A; Barberis, M; Pepe, F; Pisapia, P; Serra, N; Vigliar, E; Bellevicine, C; Fassan, M; Rugge, M; de Andrea, Ce; Lozano, Md; Basolo, F; Fontanini, G; Nikiforov, Ye; Kamel-Reid, S; da Cunha Santos, G; Nikiforova, Mn; Roy-Chowdhuri, S; Troncone, G. - In: CANCER CYTOPATHOLOGY. - ISSN 1934-662X. - (2017). [10.1002/cncy.21868]
Consistency and reproducibility of next-generation sequencing and other multigene mutational assays: A worldwide ring trial study on quantitative cytological molecular reference specimens
Malapelle U;Pisapia P;Vigliar EMethodology
;Bellevicine C;Troncone G
2017
Abstract
Molecular testing of cytological lung cancer specimens includes, beyond epidermal growth factor receptor (EGFR), emerging predictive/prognostic genomic biomarkers such as Kirsten rat sarcoma viral oncogene homolog (KRAS), neuroblastoma RAS viral [v-ras] oncogene homolog (NRAS), B-Raf proto-oncogene, serine/threonine kinase (BRAF), and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α (PIK3CA). Next-generation sequencing (NGS) and other multigene mutational assays are suitable for cytological specimens, including smears. However, the current literature reflects single-institution studies rather than multicenter experiences.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
