Marine microorganisms represent a reservoir of new promising secondary metabolites. Surface-active proteins with good emulsification activity can be isolated from fungal species that inhabit the marine environment and can be promising candidates for different biotechnological applications. In this study a novel surface-active protein, named Sap-Pc, was purified from a marine strain of Penicillium chrysogenum. The effect of salt concentration and temperature on protein production was analyzed, and a purification method was set up. The purified protein, identified as Pc13g06930, was annotated as a hypothetical protein. It was able to form emulsions, which were stable for at least one month, with an emulsification index comparable to that of other known surface-active proteins. The surface tension reduction was analyzed as function of protein concentration and a critical micellar concentration of 2 M was determined. At neutral or alkaline pH, secondary structure changes were monitored over time, concurrently with the appearance of protein precipitation. Formation of amyloid-like fibrils of SAP-Pc was demonstrated by spectroscopic and microscopic analyses. Moreover, the effect of protein concentration, a parameter affecting kinetics of fibril formation, was investigated and an on-pathway involvement of micellar aggregates during the fibril formation process was suggested.

Characterization of a surface-active protein extracted from a marine strain of penicillium chrysogenum / Cicatiello, P.; Stanzione, Ilaria; Dardano, P.; De Stefano, L.; Birolo, L.; DE CHIARO, Addolorata; Monti, D. M.; Petruk, G.; D'Errico, G.; Giardina, P.. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 20:13(2019), pp. 3242-3254. [10.3390/ijms20133242]

Characterization of a surface-active protein extracted from a marine strain of penicillium chrysogenum

Cicatiello P.;STANZIONE, ILARIA;Dardano P.;De Stefano L.;Birolo L.;DE CHIARO, ADDOLORATA;Monti D. M.;Petruk G.;D'errico G.;Giardina P.
2019

Abstract

Marine microorganisms represent a reservoir of new promising secondary metabolites. Surface-active proteins with good emulsification activity can be isolated from fungal species that inhabit the marine environment and can be promising candidates for different biotechnological applications. In this study a novel surface-active protein, named Sap-Pc, was purified from a marine strain of Penicillium chrysogenum. The effect of salt concentration and temperature on protein production was analyzed, and a purification method was set up. The purified protein, identified as Pc13g06930, was annotated as a hypothetical protein. It was able to form emulsions, which were stable for at least one month, with an emulsification index comparable to that of other known surface-active proteins. The surface tension reduction was analyzed as function of protein concentration and a critical micellar concentration of 2 M was determined. At neutral or alkaline pH, secondary structure changes were monitored over time, concurrently with the appearance of protein precipitation. Formation of amyloid-like fibrils of SAP-Pc was demonstrated by spectroscopic and microscopic analyses. Moreover, the effect of protein concentration, a parameter affecting kinetics of fibril formation, was investigated and an on-pathway involvement of micellar aggregates during the fibril formation process was suggested.
2019
Characterization of a surface-active protein extracted from a marine strain of penicillium chrysogenum / Cicatiello, P.; Stanzione, Ilaria; Dardano, P.; De Stefano, L.; Birolo, L.; DE CHIARO, Addolorata; Monti, D. M.; Petruk, G.; D'Errico, G.; Giardina, P.. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 20:13(2019), pp. 3242-3254. [10.3390/ijms20133242]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/758776
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