Pseudomonas ANT_J38B is a Gram-negative bacterium isolated from an Antarctic island. LPS was extracted using the phenol/chloroform/petroleum ether method. A mild acid hydrolysis followed by a gel filtration purification afforded the O-chain. The polysaccharide was characterized by means of chemical analyses and NMR spectroscopy. The O-chain displays a disaccharide repeating unit with the following backbone: →4)-α-L-GulpNAc3OAcAN-(1 →3)-β-D-QuipNAc-(1→.
O-specific polysaccharide structure isolated from the LPS of the Antarctic bacterium Pseudomonas ANT_J38B / Di Guida, R.; Casillo, A.; Corsaro, M. M.. - In: CARBOHYDRATE RESEARCH. - ISSN 0008-6215. - 497:(2020), p. 108125. [10.1016/j.carres.2020.108125]
O-specific polysaccharide structure isolated from the LPS of the Antarctic bacterium Pseudomonas ANT_J38B
Di Guida R.;Casillo A.;Corsaro M. M.
2020
Abstract
Pseudomonas ANT_J38B is a Gram-negative bacterium isolated from an Antarctic island. LPS was extracted using the phenol/chloroform/petroleum ether method. A mild acid hydrolysis followed by a gel filtration purification afforded the O-chain. The polysaccharide was characterized by means of chemical analyses and NMR spectroscopy. The O-chain displays a disaccharide repeating unit with the following backbone: →4)-α-L-GulpNAc3OAcAN-(1 →3)-β-D-QuipNAc-(1→.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
