Biochar-derived smoke-water exerts biological effects on nematodes, insects, and higher plants but not fungi

The pyrolysis of organic feedstock yields the solid fraction biochar, bio-oils, and a volatile fraction that can be reused for energetic purposes or technological applications in agro-ecosystems in the form of smoke-water (SW). In this study, 10 SW types were created from five organic feedstocks (i.e. cellulose, wood sawdust, olive mill residues, maize, and alfalfa litter) at two pyrolysis temperatures (i.e. 300 and 500 °C). We characterized SW using liquid chromatography (LC)–electrospray ionization–time-of-flight (TOF) mass spectrometry (MS) combined with a multi-species bioassay including five crop plants, four fungi, one root-knot nematode (Meloidogyne incognita), and the olive fly pest (Bactrocera oleae). All SW types were acidic, exhibiting a pH range of 1.9–4.6. LC-MS analysis revealed differences in the chemical profiles of SW types in relation to the organic feedstock type and pyrolysis temperature. All SW types exerted concentration-dependent effects on crops, with evident phytotoxic activity at high concentrations. Conversely, they exerted stimulatory effects when diluted with water at ratios ranging from 1:100 to 1:1000. Moreover, all SW types displayed slight or null fungitoxic activity. On the contrary, SW strongly inhibited egg hatching by M. incognita after 72 and 144 h of incubation. The strongest inhibition was found for olive mill SW, and the weakest effect was noted for alfalfa SW. Finally, the application of SW over fresh olives reversed the attraction of B. oleae adults, demonstrating a strong repellent effect toward this pest. Nevertheless, only olive mill SW consistently attracted B. oleae. In conclusion, biochar SW exhibited notable biological activities and potential applications for plant growth promotion, if opportunely diluted, and for the control of root-knot nematodes and olive fruit flies.