The acetic acid bacterium Asaia spp. was successfully detected in Anopheles arabiensis Patton, 1905, one of the major vector of human malaria in Sub-Saharan Africa. A collection of 45 Asaia isolates in cellfree media was established from 20 individuals collected from the field in Burkina Faso. 16S rRNA universal polymerase chain reaction (PCR) and specific qPCR, for the detection of Asaia spp. were performed in order to reveal the presence of different bacterial taxa associated with this insect. The isolates were typed by internal transcribed spacer-PCR, BOX-PCR, and randomly amplified polymorphic DNA-PCR, proved the presence of different Asaia in A. arabiensis.
Molecular typing of bacteria of the genus Asaia in malaria vector Anopheles arabiensis Patton, 1905 / Epis, S.; Montagna, M.; Comandatore, F.; Damiani, C.; Diabaté, A.; Daffonchio, D.; Chouaia, B.; Favia, G.. - In: JOURNAL OF ENTOMOLOGICAL AND ACAROLOGICAL RESEARCH. - ISSN 2038-324X. - 44:2(2012), pp. 33-36. [10.4081/jear.2012.e7]
Molecular typing of bacteria of the genus Asaia in malaria vector Anopheles arabiensis Patton, 1905
M. Montagna;D. Daffonchio;
2012
Abstract
The acetic acid bacterium Asaia spp. was successfully detected in Anopheles arabiensis Patton, 1905, one of the major vector of human malaria in Sub-Saharan Africa. A collection of 45 Asaia isolates in cellfree media was established from 20 individuals collected from the field in Burkina Faso. 16S rRNA universal polymerase chain reaction (PCR) and specific qPCR, for the detection of Asaia spp. were performed in order to reveal the presence of different bacterial taxa associated with this insect. The isolates were typed by internal transcribed spacer-PCR, BOX-PCR, and randomly amplified polymorphic DNA-PCR, proved the presence of different Asaia in A. arabiensis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
