In this study we investigated the transcriptome and epigenome dynamics of the tomato fruit during post-harvest in a landrace belonging to a group of tomatoes (Solanum lycopersicum L.) collectively known as "Piennolo del Vesuvio", all characterized by a long shelf-life. Expression of protein-coding genes and microRNAs as well as DNA methylation patterns and histone modifications were analysed in distinct post-harvest phases. Multi-omics data integration contributed to the elucidation of the molecular mechanisms underlying processes leading to long shelf-life. We unveiled global changes in transcriptome and epigenome. DNA methylation increased and the repressive histone mark H3K27me3 was lost as the fruit progressed from red ripe to 150 days post-harvest. Thousands of genes were differentially expressed, about half of which were potentially epi-regulated as they were engaged in at least one epi-mark change in addition to being microRNA targets in ~5% of cases. Down-regulation of the ripening regulator MADS-RIN and of genes involved in ethylene response and cell wall degradation was consistent with the delayed fruit softening. Large-scale epigenome reprogramming that occurred in the fruit during post-harvest likely contributed to delayed fruit senescence.
Multi-omics data integration provides insights into the post-harvest biology of a long shelf-life tomato landrace / Cigliano, Riccardo Aiese; Aversano, Riccardo; Di Matteo, Antonio; Palombieri, Samuela; Termolino, Pasquale; Angelini, Claudia; Bostan, Hamed; Cammareri, Maria; Consiglio, Federica Maria; Ragione, Floriana Della; Paparo, Rosa; Valkov, Vladimir Totev; Vitiello, Antonella; Carputo, Domenico; Chiusano, Maria Luisa; D'Esposito, Maurizio; Grandillo, Silvana; Matarazzo, Maria Rosaria; Frusciante, Luigi; D'Agostino, Nunzio; Conicella, Clara. - In: HORTICULTURE RESEARCH. - ISSN 2052-7276. - 9:(2022). [10.1093/hr/uhab042]
Multi-omics data integration provides insights into the post-harvest biology of a long shelf-life tomato landrace
Aversano, Riccardo;Di Matteo, Antonio;Termolino, Pasquale;Bostan, Hamed;Cammareri, Maria;Consiglio, Federica Maria;Paparo, Rosa;Carputo, Domenico;Chiusano, Maria Luisa;D'Esposito, Maurizio;Matarazzo, Maria Rosaria;Frusciante, Luigi;D'Agostino, Nunzio
;Conicella, Clara
2022
Abstract
In this study we investigated the transcriptome and epigenome dynamics of the tomato fruit during post-harvest in a landrace belonging to a group of tomatoes (Solanum lycopersicum L.) collectively known as "Piennolo del Vesuvio", all characterized by a long shelf-life. Expression of protein-coding genes and microRNAs as well as DNA methylation patterns and histone modifications were analysed in distinct post-harvest phases. Multi-omics data integration contributed to the elucidation of the molecular mechanisms underlying processes leading to long shelf-life. We unveiled global changes in transcriptome and epigenome. DNA methylation increased and the repressive histone mark H3K27me3 was lost as the fruit progressed from red ripe to 150 days post-harvest. Thousands of genes were differentially expressed, about half of which were potentially epi-regulated as they were engaged in at least one epi-mark change in addition to being microRNA targets in ~5% of cases. Down-regulation of the ripening regulator MADS-RIN and of genes involved in ethylene response and cell wall degradation was consistent with the delayed fruit softening. Large-scale epigenome reprogramming that occurred in the fruit during post-harvest likely contributed to delayed fruit senescence.File | Dimensione | Formato | |
---|---|---|---|
AieseCigliano_et_la_2022.pdf
accesso aperto
Tipologia:
Versione Editoriale (PDF)
Licenza:
Creative commons
Dimensione
1.97 MB
Formato
Adobe PDF
|
1.97 MB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.