Phytoremediation of PAH- and Cu-Contaminated Soil by Cannabis sativa L.: Preliminary Experiments on a Laboratory Scale

This study proposes the phytoremediation of phenanthrene (PHE)-, pyrene (PYR)-, and copper (Cu)-contaminated soil by Cannabis sativa L. The experimental campaign was conducted in 300 mL volume pots over a 50 d period using different initial polycyclic aromatic hydrocarbon (PAH) concentrations, i.e., 100 (PC1), 200 (PC2), and 300 (PC3) mg ƩPAHs kg−1 dry weight of soil, while maintaining a constant Cu concentration of 350 mg∙kg−1. PHE and PYR removal was 93 and 61%, 98 and 48%, and 97 and 36% in PC1, PC2, and PC3, respectively, in the greenhouse condition. The highest Cu extraction amounted to 58 mg∙kg−1. In general, the growth of C. sativa L. under the PC1, PC2, and PC3 conditions decreased by approximately 25, 65, and 71% (dry biomass), respectively, compared to the uncontaminated control. The present study is aimed at highlighting the phytoremediation potential of C. sativa L. and providing the preliminary results necessary for future field-scale investigations.