Design of nanostructured coating to prevent biofilm formation on surfaces

Biofilms are surface-attached microbial organizations, where microbial species are enclosed in an exo-polysaccharides matrix. Its removal/prevention from surfaces represents an important challenge and a relevant issue in many fields. In this light, antimicrobial peptides AMPs) represent good candidates to conventional antibiotics due to their selectivity towards targets (cell membranes), rapid action and low tendency to induce bacterial resistance. In our project we are investigating novel techniques to realise nanostructured coatings on surfaces, in order to prevent bacterial adhesion and biofilm formation. We are focusing our attention on a well-known antimicrobial peptide (Magainin-2). Two bacteria models have been considered: E.coli and P. fluorescens. Our goal is to optimize AMPs grafting on surfaces by means of treatment methodologies based on Oxygen plasma technologies. In this work we present a methodology to investigate anti-biofilm efficiency of the nano-functionalized surfaces, compared with the case of the raw surfaces. Biofilm morphologies can be measured using Confocal Laser Scanning Microscopy and image analysis techniques to quantify its structure by measuring quantitative morphological parameters.