: Cold stress represents one of the major constraints for agricultural systems and crops productivity, inducing a wide range of negative effects. Particularly, long-term cold stress affects lipid metabolism, modifying the lipids/proteins ratio, the levels of phospholipids and glycolipids, and increasing lipids' unsaturation in bio-membranes. Glucose-6-phosphate dehydrogenase (G6PDH) reported prominent roles as NADPH suppliers in response to oxidative perturbations. Cytosolic G6PDH was suggested as the main isoform involved in cold stress response, while a down-regulation of the chloroplastic P1-G6PDH was observed. We thus investigated an Arabidopsis mutant defective for the P1-G6PDH (KO-P1) using integrated approaches to verify a possible role of this isoform in low temperature tolerance. KO-P1 genotype showed an improved tolerance to cold stress, highlighting a better photosynthetic efficiency, a reduction in stress markers content and a different regulation of genes involved in stress response. Intriguingly, the lack of P1-G6PDH induced modification in the levels of the main fatty acid and lipid species affecting the morphology of chloroplasts and mitochondria, which was restored under cold. Globally, these results indicate a priming effect induced by the absence of P1-G6PDH able to improve the tolerance to abiotic stress. Our results suggest novel and specific abilities of P1-G6PDH, highlighting its central role in different aspects of plant physiology and metabolism.

Lack of Arabidopsis chloroplastic glucose-6-phosphate dehydrogenase 1 (G6PD1) affects lipid synthesis during cold stress response / Landi, Simone; Vitale, Ermenegilda; Lanzilli, Mariamichela; Arena, Carmen; D'Ippolito, Giuliana; Fontana, Angelo; Esposito, Sergio. - In: PLANT SCIENCE. - ISSN 0168-9452. - 349:(2024). [10.1016/j.plantsci.2024.112260]

Lack of Arabidopsis chloroplastic glucose-6-phosphate dehydrogenase 1 (G6PD1) affects lipid synthesis during cold stress response

Landi, Simone
Primo
;
Vitale, Ermenegilda;Lanzilli, Mariamichela;Arena, Carmen;Fontana, Angelo;Esposito, Sergio
Ultimo
2024

Abstract

: Cold stress represents one of the major constraints for agricultural systems and crops productivity, inducing a wide range of negative effects. Particularly, long-term cold stress affects lipid metabolism, modifying the lipids/proteins ratio, the levels of phospholipids and glycolipids, and increasing lipids' unsaturation in bio-membranes. Glucose-6-phosphate dehydrogenase (G6PDH) reported prominent roles as NADPH suppliers in response to oxidative perturbations. Cytosolic G6PDH was suggested as the main isoform involved in cold stress response, while a down-regulation of the chloroplastic P1-G6PDH was observed. We thus investigated an Arabidopsis mutant defective for the P1-G6PDH (KO-P1) using integrated approaches to verify a possible role of this isoform in low temperature tolerance. KO-P1 genotype showed an improved tolerance to cold stress, highlighting a better photosynthetic efficiency, a reduction in stress markers content and a different regulation of genes involved in stress response. Intriguingly, the lack of P1-G6PDH induced modification in the levels of the main fatty acid and lipid species affecting the morphology of chloroplasts and mitochondria, which was restored under cold. Globally, these results indicate a priming effect induced by the absence of P1-G6PDH able to improve the tolerance to abiotic stress. Our results suggest novel and specific abilities of P1-G6PDH, highlighting its central role in different aspects of plant physiology and metabolism.
2024
Lack of Arabidopsis chloroplastic glucose-6-phosphate dehydrogenase 1 (G6PD1) affects lipid synthesis during cold stress response / Landi, Simone; Vitale, Ermenegilda; Lanzilli, Mariamichela; Arena, Carmen; D'Ippolito, Giuliana; Fontana, Angelo; Esposito, Sergio. - In: PLANT SCIENCE. - ISSN 0168-9452. - 349:(2024). [10.1016/j.plantsci.2024.112260]
File in questo prodotto:
File Dimensione Formato  
2024 - Lack of Arabidopsis chloroplastic glucose-6-phosphate dehydrogenase 1 affect lipid synthesis during cold stress.pdf

solo utenti autorizzati

Tipologia: Versione Editoriale (PDF)
Licenza: Non specificato
Dimensione 4.67 MB
Formato Adobe PDF
4.67 MB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/983634
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 0
  • ???jsp.display-item.citation.isi??? 0
social impact