The N-capping region of an α-helix is a short N-terminal amino acid stretch that contributes to nucleate and stabilize the helical structure. In the VEGF mimetic helical peptide QK, the N-capping region was previously demonstrated to be a key factor of QK helical folding. In this paper, we explored the effect of the chiral inversion of the N-capping sequence on QK folding, performing conformational analysis in solution by circular dichroism and NMR spectroscopy. The effect of such a modification on QK stability in serum and the proliferative effect were also evaluated.
Switching the N-Capping Region from all-L to all-D Amino Acids in a VEGF Mimetic Helical Peptide / De Rosa, L.; Diana, D.; Capasso, D.; Stefania, R.; Di Stasi, R.; Fattorusso, R.; D'Andrea, L. D.. - In: MOLECULES. - ISSN 1420-3049. - 27:20(2022). [10.3390/molecules27206982]
Switching the N-Capping Region from all-L to all-D Amino Acids in a VEGF Mimetic Helical Peptide
Fattorusso R.;D'Andrea L. D.
2022
Abstract
The N-capping region of an α-helix is a short N-terminal amino acid stretch that contributes to nucleate and stabilize the helical structure. In the VEGF mimetic helical peptide QK, the N-capping region was previously demonstrated to be a key factor of QK helical folding. In this paper, we explored the effect of the chiral inversion of the N-capping sequence on QK folding, performing conformational analysis in solution by circular dichroism and NMR spectroscopy. The effect of such a modification on QK stability in serum and the proliferative effect were also evaluated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
