The advanced-PRF+ (A-PRF+) is a platelet concentrate, showing a higher concentration of growth factors, an increased number of cells and looser structure of the fibrin clot than leukocyte-PRF. A high variability in the size of PRF associated with patients, haematological features and centrifugation protocols was reported. The aims of this study were to evaluate the feasibility of A-PRF+ production in the field and the correlation between hae- matological parameters, macroscopic and microscopic features in equine A-PRF+. Samples from twenty Stan- dardbred horses (3–7 years) were harvested with glass tubes without anticoagulants, previously heated at 37 ◦C. Blood samples were centrifugated at 1300 rpm for 8 min with a fixed-angle centrifuge and a horizontal centrifuge in the field, at a temperature of 15–17 ◦C. Clots were measured and placed on the Wound Box® for a 2-min compression. Membranes were measured and fixed in 10% formalin for histological examination. Clot and membrane surface did not differ between sex and centrifuge. Haematological parameters did not show a sig- nificant correlation to clot and membrane size. Membranes obtained from both centrifugation protocols showed a loose fibrin structure and cells evenly distributed throughout the clot. Tubes’ warming was effective to obtain A-PRF+ clots from all samples, regardless the environmental temperature. Further studies are needed to evaluate the influence of other blood molecules on the A-PRF+ structure and size

Influence of haematological parameters on size of the advanced platelet-rich fibrin+ (A-PRF+) in the horse / Montano, Chiara; DE CHIARA, Mariaelena; Crisci, Alessandro; DE BIASE, Davide; Ciuca, Lavinia; Maurelli, MARIA PAOLA; Lamagna, Barbara; DEL PRETE, Chiara; Fabiana, Flagiello; Pasolini, MARIA PIA. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - 177:(2024), p. 105367. [10.1016/j.rvsc.2024.105367]

Influence of haematological parameters on size of the advanced platelet-rich fibrin+ (A-PRF+) in the horse

Chiara, Montano
Primo
;
Mariaelena, de Chiara
Secondo
;
Alessandro, Crisci;Davide, De Biase;Lavinia, Ciuca;Paola, Maurelli Maria;Barbara, Lamagna;Chiara, Del Prete;Pia, Pasolini Maria
Ultimo
2024

Abstract

The advanced-PRF+ (A-PRF+) is a platelet concentrate, showing a higher concentration of growth factors, an increased number of cells and looser structure of the fibrin clot than leukocyte-PRF. A high variability in the size of PRF associated with patients, haematological features and centrifugation protocols was reported. The aims of this study were to evaluate the feasibility of A-PRF+ production in the field and the correlation between hae- matological parameters, macroscopic and microscopic features in equine A-PRF+. Samples from twenty Stan- dardbred horses (3–7 years) were harvested with glass tubes without anticoagulants, previously heated at 37 ◦C. Blood samples were centrifugated at 1300 rpm for 8 min with a fixed-angle centrifuge and a horizontal centrifuge in the field, at a temperature of 15–17 ◦C. Clots were measured and placed on the Wound Box® for a 2-min compression. Membranes were measured and fixed in 10% formalin for histological examination. Clot and membrane surface did not differ between sex and centrifuge. Haematological parameters did not show a sig- nificant correlation to clot and membrane size. Membranes obtained from both centrifugation protocols showed a loose fibrin structure and cells evenly distributed throughout the clot. Tubes’ warming was effective to obtain A-PRF+ clots from all samples, regardless the environmental temperature. Further studies are needed to evaluate the influence of other blood molecules on the A-PRF+ structure and size
2024
Influence of haematological parameters on size of the advanced platelet-rich fibrin+ (A-PRF+) in the horse / Montano, Chiara; DE CHIARA, Mariaelena; Crisci, Alessandro; DE BIASE, Davide; Ciuca, Lavinia; Maurelli, MARIA PAOLA; Lamagna, Barbara; DEL PRETE, Chiara; Fabiana, Flagiello; Pasolini, MARIA PIA. - In: RESEARCH IN VETERINARY SCIENCE. - ISSN 0034-5288. - 177:(2024), p. 105367. [10.1016/j.rvsc.2024.105367]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/987995
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