We have analysed the microRNA (miR) profile of ATC in comparison to the normal thyroid using a microarray (miRNACHIP microarray). We found an aberrant miR expression profile that clearly differentiates ATC from normal thyroid tissues and from PTC analysed in previous studies. In particular, a significant decrease in miR-30d, miR-125b, miR-26a and miR-30a-5p was detected in ATC in comparison to normal thyroid tissue. These results were further confirmed by northern blots, quantitative reverse transcription-PCR analyses and in situ hybridization. The overexpression of these four miRs in two human ATC-derived cell lines suggests a critical role of miR-125b and miR-26a downregulation in thyroid carcinogenesis, since a cell growth inhibition was achieved. Conversely, no effect on cell growth was observed after the overexpression of miR-30d and miR-30a-5p in the same cells. In conclusion, these data indicate a miR signature associated with ATC and suggest the miR deregulation as an important event in thyroid cell transformation.
Specific microRNAs are downregulated in human thyroid anaplastic carcinomas / Visone, R; Pallante, P; Vecchione, A; Cirombella, R; Ferracin, M; Ferraro, A; Volinia, S; Coluzzi, S; Leone, Vincenza; Borbone, E; Liu, Cg; Petrocca, F; Troncone, Giancarlo; Calin, Ga; Scarpa, A; Colato, C; Tallini, G; Santoro, Massimo; Croce, Cm; Fusco, Alfredo. - In: ONCOGENE. - ISSN 0950-9232. - STAMPA. - 26:54(2007), pp. 7590-7595. [10.1038/sj.onc.1210564]
Specific microRNAs are downregulated in human thyroid anaplastic carcinomas.
LEONE, Vincenza;TRONCONE, GIANCARLO;SANTORO, MASSIMO;FUSCO, ALFREDO
2007
Abstract
We have analysed the microRNA (miR) profile of ATC in comparison to the normal thyroid using a microarray (miRNACHIP microarray). We found an aberrant miR expression profile that clearly differentiates ATC from normal thyroid tissues and from PTC analysed in previous studies. In particular, a significant decrease in miR-30d, miR-125b, miR-26a and miR-30a-5p was detected in ATC in comparison to normal thyroid tissue. These results were further confirmed by northern blots, quantitative reverse transcription-PCR analyses and in situ hybridization. The overexpression of these four miRs in two human ATC-derived cell lines suggests a critical role of miR-125b and miR-26a downregulation in thyroid carcinogenesis, since a cell growth inhibition was achieved. Conversely, no effect on cell growth was observed after the overexpression of miR-30d and miR-30a-5p in the same cells. In conclusion, these data indicate a miR signature associated with ATC and suggest the miR deregulation as an important event in thyroid cell transformation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
