Abstract The effects of cryoprotectants, cooling rate and freezing on the mussel Mytilus galloprovincialis sperm were evaluated. At the end of each step of the experimental protocol, motility and fertilization ability of sperm were analyzed, compared to fresh semen. Five cryoprotectants were tested in their toxicity level: dimethylsulfoxide, ethylene glycol, 1-2 propylene glycol at 5%, 7%, 10%, 15% and 20% concentration; glycerol and methanol at concentration of 5%, 7% and 10%. The incubation times were 10, 20 and 30 min at 20 ± 1 _C. Only dimethylsulfoxide, ethylene glycol and 1-2 propylene glycol at 5%, 7% and 10% were chosen for the following pre-freezing step. Five adaptation/chilling rates were analyzed: 10 min at 20 ± 1, _2, _1, _0.5 and _0.25 _C/min and the last one was used for testing the best freezing Thawing results showed that M. galloprovincialis sperm are very sensitive to rapid pre-freezing and freezing protocols and only a slow procedure assured good motility and fertilization percentage.

Cryopreservation of the Mediterranean mussel (Mytilus galloprovincialis) spermatozoa / DI MATTEO, O.; Langellotti, A. L.; Masullo, P.; Sansone, Giovanni. - In: CRYOBIOLOGY. - ISSN 0011-2240. - STAMPA. - 58:(2009), pp. 145-150.

Cryopreservation of the Mediterranean mussel (Mytilus galloprovincialis) spermatozoa

A. L. LANGELLOTTI;SANSONE, GIOVANNI
2009

Abstract

Abstract The effects of cryoprotectants, cooling rate and freezing on the mussel Mytilus galloprovincialis sperm were evaluated. At the end of each step of the experimental protocol, motility and fertilization ability of sperm were analyzed, compared to fresh semen. Five cryoprotectants were tested in their toxicity level: dimethylsulfoxide, ethylene glycol, 1-2 propylene glycol at 5%, 7%, 10%, 15% and 20% concentration; glycerol and methanol at concentration of 5%, 7% and 10%. The incubation times were 10, 20 and 30 min at 20 ± 1 _C. Only dimethylsulfoxide, ethylene glycol and 1-2 propylene glycol at 5%, 7% and 10% were chosen for the following pre-freezing step. Five adaptation/chilling rates were analyzed: 10 min at 20 ± 1, _2, _1, _0.5 and _0.25 _C/min and the last one was used for testing the best freezing Thawing results showed that M. galloprovincialis sperm are very sensitive to rapid pre-freezing and freezing protocols and only a slow procedure assured good motility and fertilization percentage.
2009
Cryopreservation of the Mediterranean mussel (Mytilus galloprovincialis) spermatozoa / DI MATTEO, O.; Langellotti, A. L.; Masullo, P.; Sansone, Giovanni. - In: CRYOBIOLOGY. - ISSN 0011-2240. - STAMPA. - 58:(2009), pp. 145-150.
File in questo prodotto:
File Dimensione Formato  
341077 -2009 Cryopreservation of the Mediterranean mussel (Mytilus galloprovincialis).pdf

non disponibili

Tipologia: Documento in Post-print
Licenza: Accesso privato/ristretto
Dimensione 769.93 kB
Formato Adobe PDF
769.93 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/341077
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact