The composition of an artificial medium and technical procedures used for in vitro rearing of the endophagous larval parasitoid Cardiochiles nigriceps Viereck (Hymenoptera, Braconidae), from post-germ band egg to the 2nd instar larva, are described. Amino acids, carbohydrates, salts, and vitamins were supplied in defined amounts as an aqueous solution which, when supplemented with 20 mg/ml of bovine albumin, 5 mg/ml of lactalbumin (enzymatic hydrolysate), 20 % (v/v) fetal bovine serum, 20 % (v/v) milk and 10% (v/v) chicken egg yolk, allowed for parasitoid growth and molting to the 2nd instar. Molting to the final instar was never observed.
Preliminary-results On Invitro Rearing of the Endoparasitoid Cardiochiles-nigriceps From Egg To 2nd Instar / Pennacchio, Francesco; S. B., Vinson; E., Tremblay. - In: ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA. - ISSN 0013-8703. - STAMPA. - 64:(1992), pp. 209-216.
Preliminary-results On Invitro Rearing of the Endoparasitoid Cardiochiles-nigriceps From Egg To 2nd Instar
PENNACCHIO, FRANCESCO;
1992
Abstract
The composition of an artificial medium and technical procedures used for in vitro rearing of the endophagous larval parasitoid Cardiochiles nigriceps Viereck (Hymenoptera, Braconidae), from post-germ band egg to the 2nd instar larva, are described. Amino acids, carbohydrates, salts, and vitamins were supplied in defined amounts as an aqueous solution which, when supplemented with 20 mg/ml of bovine albumin, 5 mg/ml of lactalbumin (enzymatic hydrolysate), 20 % (v/v) fetal bovine serum, 20 % (v/v) milk and 10% (v/v) chicken egg yolk, allowed for parasitoid growth and molting to the 2nd instar. Molting to the final instar was never observed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
