The aim of this preliminary investigation was to assess whether human peripheral blood lymphocytes which have been pre-exposed to non-ionizing radiofrequency fields exhibit an adaptive response (AR) by resisting the induction of genetic damage from subsequent exposure to ionizing radiation. Peripheral blood lymphocytes from four healthy donors were stimulated with phytohemagglutinin for 24 h and then exposed for 20 h to 1950 MHz radiofrequency fields (RF, adaptive dose, AD) at an average specific absorption rate of 0.3 W/kg. At 48 h, the cells were subjected to a challenge dose (CD) of 1.0 or 1.5 Gy X-irradiation (XR, challenge dose, CD). After a 72 h total culture period, cells were collected to examine the incidence of micronuclei (MN). There was a significant decrease in the number of MN in lymphocytes exposed to RF + XR (AD + CD) as compared with those subjected to XR alone (CD). These observations thus suggested a RF-induced AR and induction of resistance to subsequent damage from XR. There was variability between the donors in RF-induced AR. The data reported in our earlier investigations also indicated a similar induction of AR in human blood lymphocytes that had been pre-exposed to RF (AD) and subsequently treated with a chemical mutagen, mitomycin C (CD). Since XR and mitomycin-C induce different kinds of lesions in cellular DNA, further studies are required to understand the mechanism(s) involved in the RF-induced adaptive response.

Adaptive Response in Human Blood Lymphocytes Exposed to Radiofrequency Fields: Resistance to Ionizing Radiation-Induced Damage / Anna, Sannino; Olga, Zeni; Stefania, Romeo; Massa, Rita; Giancarlo, Gialanella; Grossi, Gianfranco; Manti, Lorenzo; Vijayalaxmi, ; Maria Rosaria, Scarfì. - In: JOURNAL OF RADIATION RESEARCH. - ISSN 0449-3060. - 55:(2014), pp. 210-217. [10.1093/jrr/rrt106]

Adaptive Response in Human Blood Lymphocytes Exposed to Radiofrequency Fields: Resistance to Ionizing Radiation-Induced Damage

MASSA, RITA;GROSSI, GIANFRANCO;MANTI, LORENZO;
2014

Abstract

The aim of this preliminary investigation was to assess whether human peripheral blood lymphocytes which have been pre-exposed to non-ionizing radiofrequency fields exhibit an adaptive response (AR) by resisting the induction of genetic damage from subsequent exposure to ionizing radiation. Peripheral blood lymphocytes from four healthy donors were stimulated with phytohemagglutinin for 24 h and then exposed for 20 h to 1950 MHz radiofrequency fields (RF, adaptive dose, AD) at an average specific absorption rate of 0.3 W/kg. At 48 h, the cells were subjected to a challenge dose (CD) of 1.0 or 1.5 Gy X-irradiation (XR, challenge dose, CD). After a 72 h total culture period, cells were collected to examine the incidence of micronuclei (MN). There was a significant decrease in the number of MN in lymphocytes exposed to RF + XR (AD + CD) as compared with those subjected to XR alone (CD). These observations thus suggested a RF-induced AR and induction of resistance to subsequent damage from XR. There was variability between the donors in RF-induced AR. The data reported in our earlier investigations also indicated a similar induction of AR in human blood lymphocytes that had been pre-exposed to RF (AD) and subsequently treated with a chemical mutagen, mitomycin C (CD). Since XR and mitomycin-C induce different kinds of lesions in cellular DNA, further studies are required to understand the mechanism(s) involved in the RF-induced adaptive response.
2014
Adaptive Response in Human Blood Lymphocytes Exposed to Radiofrequency Fields: Resistance to Ionizing Radiation-Induced Damage / Anna, Sannino; Olga, Zeni; Stefania, Romeo; Massa, Rita; Giancarlo, Gialanella; Grossi, Gianfranco; Manti, Lorenzo; Vijayalaxmi, ; Maria Rosaria, Scarfì. - In: JOURNAL OF RADIATION RESEARCH. - ISSN 0449-3060. - 55:(2014), pp. 210-217. [10.1093/jrr/rrt106]
File in questo prodotto:
File Dimensione Formato  
adaptive response sannino et al 2014.pdf

accesso aperto

Tipologia: Documento in Post-print
Licenza: Dominio pubblico
Dimensione 200.42 kB
Formato Adobe PDF
200.42 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/573518
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 53
  • ???jsp.display-item.citation.isi??? 43
social impact