Brain-derived neurotrophic factor (BDNF) is an important neuroprotective factor belonging to the neurotro-phin family whose effects are mediated by TrkB receptor. This functional system is involved in the biology of the vertebrate retina. BDNF exerts its survival-promoting effects on retinal photoreceptors and is critically in-volved in a variety of retinal degenerative diseases in animal models. It has been suggested also to aid in reti-nal recovery after reattachment and in ganglion cell regeneration following optic nerve injury. Recently, the teleost Nothobranchius furzeri has emerged as a model for neurobiological and age research, due to the ex-ceptionally short lifespan, age-dependent cognitive/behavioral decline and expression of age-related bio-markers. The aim of this study is to investigate BDNF pattern of expression in the retina of the N. furzeri. The occurrence of BDNF was detected by in situ hybridization (ISH) and immunohistochemistry (ICH), by employ-ing respectively a riboprobe and an antibody mapping an epitope of an internal region of BDNF.
Brain derived neurotrophic factor in the retina of Nothobranchius furzeri / Gatta, Claudia; Castaldo, Luciana; Cellerino, A; DE GIROLAMO, Paolo; Lucini, Carla; D'Angelo, Livia. - uninco:(2013), pp. 8-8. (Intervento presentato al convegno IX CONGRESSO NAZIONALE tenutosi a Roam Accademia dei Lincei nel 23/24 maggio 2013).
Brain derived neurotrophic factor in the retina of Nothobranchius furzeri
GATTA, CLAUDIA;CASTALDO, LUCIANA;DE GIROLAMO, PAOLO;LUCINI, CARLA;D'ANGELO, LIVIA
2013
Abstract
Brain-derived neurotrophic factor (BDNF) is an important neuroprotective factor belonging to the neurotro-phin family whose effects are mediated by TrkB receptor. This functional system is involved in the biology of the vertebrate retina. BDNF exerts its survival-promoting effects on retinal photoreceptors and is critically in-volved in a variety of retinal degenerative diseases in animal models. It has been suggested also to aid in reti-nal recovery after reattachment and in ganglion cell regeneration following optic nerve injury. Recently, the teleost Nothobranchius furzeri has emerged as a model for neurobiological and age research, due to the ex-ceptionally short lifespan, age-dependent cognitive/behavioral decline and expression of age-related bio-markers. The aim of this study is to investigate BDNF pattern of expression in the retina of the N. furzeri. The occurrence of BDNF was detected by in situ hybridization (ISH) and immunohistochemistry (ICH), by employ-ing respectively a riboprobe and an antibody mapping an epitope of an internal region of BDNF.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
