A novel electrochemical method for measuring transglutaminase activity was reported. This approach was based on the patterning of gold electrodes with a mixed self-assembled monolayer of perthiolated β-cyclodextrin and 1-octanethiol. The proper functionalization of β-cyclodextrin with primary amino groups allowed it to act as amino-donor substrates for transglutaminase and molecular nanopores for the enzyme-controlled diffusion of Fe(CN) 6 3 -/4 - to the electrode surface. Voltamperometric measurements allowed detection of transglutaminase in the range of 1.9-37 mU/mL with a sensitivity of 1.42 nA μL U- 1 s- 1.
Gold surface patterned with cyclodextrin-based molecular nanopores for electrochemical assay of transglutaminase activity / Iñigo, Fernàndez; Elena, Araque; Paloma Martìnez, Ruiz; DI PIERRO, Prospero; Reynaldo, Villalonga; José M., Pingarròn. - In: ELECTROCHEMISTRY COMMUNICATIONS. - ISSN 1388-2481. - 40:(2014), pp. 13-16. [10.1016/j.elecom.2013.12.014]
Gold surface patterned with cyclodextrin-based molecular nanopores for electrochemical assay of transglutaminase activity
DI PIERRO, PROSPERO;
2014
Abstract
A novel electrochemical method for measuring transglutaminase activity was reported. This approach was based on the patterning of gold electrodes with a mixed self-assembled monolayer of perthiolated β-cyclodextrin and 1-octanethiol. The proper functionalization of β-cyclodextrin with primary amino groups allowed it to act as amino-donor substrates for transglutaminase and molecular nanopores for the enzyme-controlled diffusion of Fe(CN) 6 3 -/4 - to the electrode surface. Voltamperometric measurements allowed detection of transglutaminase in the range of 1.9-37 mU/mL with a sensitivity of 1.42 nA μL U- 1 s- 1.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
