Diolein based non-targeted theranostic nanoparticles (DO-NPs) containing 10%wt of the amphiphilic Gadolinium complex (C18)2DTPA(Gd), and targeted NPs, obtained by introducing growing amounts (3%wt, 6%wt or 10%wt) of (C18)2-Peg3000-FA in the sample composition, have been studied for their in vitro and in vivo properties. Cellular binding was studied by ICP-MS analysis of the Gadolinium content and by Surface Plasmon Resonance (SPR) assays. The best formulation in terms of selectivity towards IGROV-1 cells with respect to non-targeted DO-NPs, was that containing 3% (C18)2Peg3000-FA (P<0.01). Cytotoxic studies and confocal microscopy analysis of IGROV-1 cells indicate high selective properties of the targeted doxorubicin (DOX) loaded NPs. Nanoparticles described here represent the first example in which a targeted carrier characterized by a stable foamy mesophase, provided by the Diolein component, combine the therapeutic effect due to the anticancer drug doxorubicin, with the imaging properties provided by paramagnetic gadolinium complexes for MRI. As evidenced by T1w and T2w MRI images and by the in vivo antitumor effect in IGROV-1 tumor-bearing mice, DO-NP3-FA/DOX provides very high therapeutic efficacy with a tumor growth regression of 80% and 50% higher as compared to the mice treated with saline solution and with Doxil, respectively.
Diolein based nanostructures as targeted theranostics / Accardo, Antonella; Arena, Francesca; Gianolio, Eliana; Marasco, Daniela; Ringhieri, Paola; Boffa, Cinzia; Bardini, Paola; Aime, Silvio; Morelli, Giancarlo. - In: JOURNAL OF BIOMEDICAL NANOTECHNOLOGY. - ISSN 1550-7033. - 12:5(2016), pp. 1076-1088. [10.1166/jbn.2016.2212]
Diolein based nanostructures as targeted theranostics
ACCARDO, ANTONELLA;ARENA, FRANCESCA;MARASCO, DANIELA;RINGHIERI, PAOLA;MORELLI, GIANCARLO
2016
Abstract
Diolein based non-targeted theranostic nanoparticles (DO-NPs) containing 10%wt of the amphiphilic Gadolinium complex (C18)2DTPA(Gd), and targeted NPs, obtained by introducing growing amounts (3%wt, 6%wt or 10%wt) of (C18)2-Peg3000-FA in the sample composition, have been studied for their in vitro and in vivo properties. Cellular binding was studied by ICP-MS analysis of the Gadolinium content and by Surface Plasmon Resonance (SPR) assays. The best formulation in terms of selectivity towards IGROV-1 cells with respect to non-targeted DO-NPs, was that containing 3% (C18)2Peg3000-FA (P<0.01). Cytotoxic studies and confocal microscopy analysis of IGROV-1 cells indicate high selective properties of the targeted doxorubicin (DOX) loaded NPs. Nanoparticles described here represent the first example in which a targeted carrier characterized by a stable foamy mesophase, provided by the Diolein component, combine the therapeutic effect due to the anticancer drug doxorubicin, with the imaging properties provided by paramagnetic gadolinium complexes for MRI. As evidenced by T1w and T2w MRI images and by the in vivo antitumor effect in IGROV-1 tumor-bearing mice, DO-NP3-FA/DOX provides very high therapeutic efficacy with a tumor growth regression of 80% and 50% higher as compared to the mice treated with saline solution and with Doxil, respectively.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.