Statistical optimization of levan: Influence of the parameter on levan structure and angiotensin I-converting enzyme inhibitory

Based on Plackett–Burman design, steepest ascent method, and Box–Behnken design, statistical optimization for B. subtilis AF17 for levan production was carried out. Sucrose, tryptone and initial pH were found to be the most significant parameter (P < 0.05) for levan production. Result showed that the optimum condition was sucrose 162.5 g/L, tryptone 10 g/L, initial pH 7 and maximum yield was 7.9 ± 0.18 g/L in 72 h fermentation. Purified levan was characterized using various physicochemical techniques such as GC–MS, 1H NMR, 13C NMR spectroscopy and SEC/TDA. Based on this data, the structure of levan was independent of initial culture conditions. The biomedical potential of the isolated Bacillus subtilis A17 levan for its angiotensin-I converting enzyme (ACE) inhibition activities was exploited in vitro. Interestingly, levan possessed an important angiotensin I converting enzyme (ACE) inhibitory 81.1 ± 4.1% at 4 mg/mL. The overall, data suggested that levan presents a promising natural source of antihypertensive agents.