Identification of coagulase negative Staphylococci isolated from dairy products using molecular methods

Thirty-one coagulase negative Staphylococcus strains (CNS) and two strains of coagulase positive Staphylococcus (CPS) isolated from dairy products were identified using phenotypic and genotypic methods. All the strains were well identified by genotypic methods supporting the usefulness of these tools. Intergenic Spacer-polymerase chain reaction (ITS-PCR) based on polymorphism of the 16-23S rRNA intergenic spacer region and Pulse Field Gel Electrophoresis based on the analysis of DNA macrorestriction fragments appeared to be efficient methods for a further extensive analysis of CNS of staphylococcal isolates at the species and subspecies level. Parole chiave coagulase negative strains of Staphylococcus, dairy products, intergenic Spacer-polymerase chain reaction (ITS-PCR), pulse field gel electrophoresis (PFGE),