Three different techniques for bacterial mating were applied to wild type and culture collection strains of Lactococcus lactis harbouring transposons: direct plate conjugation, filter mating and mating on milk agar. Efficiencies and frequencies of transfer were compared. Transconjugants were characterized by marker properties and molecular assays. Transposon-coded Suc(+) Nis(+) phenotype as well as Suc(+) Bac(+) Nis(-) phenotype were transferred with frequencies ranging between 10(-9) and 10(-6). Milk agar plate mating was the best technique for obtaining gene transfer events involving wild type lactococci.
Conditions for conjugative transposon transfer in Lactococcus lactis / Blaiotta, Giuseppe; Ercolini, Danilo; E., Simeoli; Moschetti, Giancarlo; Villani, Francesco. - In: LETTERS IN APPLIED MICROBIOLOGY. - ISSN 0266-8254. - STAMPA. - 31:(2000), pp. 343-348.
Conditions for conjugative transposon transfer in Lactococcus lactis
BLAIOTTA, GIUSEPPE;ERCOLINI, DANILO;MOSCHETTI, GIANCARLO;VILLANI, FRANCESCO
2000
Abstract
Three different techniques for bacterial mating were applied to wild type and culture collection strains of Lactococcus lactis harbouring transposons: direct plate conjugation, filter mating and mating on milk agar. Efficiencies and frequencies of transfer were compared. Transconjugants were characterized by marker properties and molecular assays. Transposon-coded Suc(+) Nis(+) phenotype as well as Suc(+) Bac(+) Nis(-) phenotype were transferred with frequencies ranging between 10(-9) and 10(-6). Milk agar plate mating was the best technique for obtaining gene transfer events involving wild type lactococci.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
