The heterogeneity of ovine beta-casein, apparent after discontinuous gel electrophoresis at alkaline pH and gel isoelectric focusing either by staining with Coomassie blue or immunoblotting, was investigated in detail. Electrospray mass spectrometry was used to characterize ovine casein by direct examination of whole casein samples. beta-Casein in individual milks consisted of several components which were characterized through the accurate determination of their molecular masses. It was demonstrated that protein species occurring simultaneously in individual ovine milks differed mainly in the number of phosphate groups.
DISCRETE PHOSPHORYLATION GENERATES THE ELECTROPHORETIC HETEROGENEITY OF OVINE BETA-CASEIN / Chianese, Lina; Garro, Giuseppina; Ferranti, Pasquale; A., Malorni; Addeo, Francesco; A., Rabasco; P. M., Pons. - In: THE JOURNAL OF DAIRY RESEARCH. - ISSN 0022-0299. - STAMPA. - 62:1(1995), pp. 89-100.
DISCRETE PHOSPHORYLATION GENERATES THE ELECTROPHORETIC HETEROGENEITY OF OVINE BETA-CASEIN
CHIANESE, LINA;GARRO, GIUSEPPINA;FERRANTI, PASQUALE;ADDEO, FRANCESCO;
1995
Abstract
The heterogeneity of ovine beta-casein, apparent after discontinuous gel electrophoresis at alkaline pH and gel isoelectric focusing either by staining with Coomassie blue or immunoblotting, was investigated in detail. Electrospray mass spectrometry was used to characterize ovine casein by direct examination of whole casein samples. beta-Casein in individual milks consisted of several components which were characterized through the accurate determination of their molecular masses. It was demonstrated that protein species occurring simultaneously in individual ovine milks differed mainly in the number of phosphate groups.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
